ccr7 chemokine  (MetaStat Inc)

Journal: Biomaterials Research

Article Title: Biomimetic Gradient Microporous Scaffold with a Triply Periodic Minimal Surface Enhances Osseointegration by Modulating Macrophage Polarization

doi: 10.34133/bmr.0266

Figure Lengend Snippet: In vivo immune modulation evaluation. (A) Immunofluorescence staining of C-C motif chemokine receptor 7 (CCR7, M1, green) and mannose receptor (CD206, M2, red). (B) Quantitative analysis of CCR7 and CD206 expression. Data are presented as mean ± SD ( n = 3). * indicates significant differences (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

Article Snippet: Rabbit anti-C-C motif chemokine receptor 7 (anti-CCR7) antibody (M1 marker, Boster, China) and rabbit anti-mannose receptor (anti-CD206) antibody (M2 marker, Boster, China) were used to stain the samples, as these are well-known markers for M1 and M2 macrophage phenotypes, respectively.

Techniques: In Vivo, Immunofluorescence, Staining, Expressing

Journal: Biomaterials Research

Article Title: Biomimetic Gradient Microporous Scaffold with a Triply Periodic Minimal Surface Enhances Osseointegration by Modulating Macrophage Polarization

doi: 10.34133/bmr.0266

Figure Lengend Snippet: Immunohistochemistry (IHC) staining images of bone tissue surrounding the scaffolds after 4 weeks of implantation. (A) IHC staining images of osteogenesis-related markers (BMP-2 and RUNX2) and macrophage polarization markers (CCR7 [M1] and Arg-1 [M2]). (B) Quantitative analysis of various markers from IHC staining images. Data are presented as mean ± SD ( n = 3). * indicates significant differences (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

Article Snippet: Rabbit anti-C-C motif chemokine receptor 7 (anti-CCR7) antibody (M1 marker, Boster, China) and rabbit anti-mannose receptor (anti-CD206) antibody (M2 marker, Boster, China) were used to stain the samples, as these are well-known markers for M1 and M2 macrophage phenotypes, respectively.

Techniques: Immunohistochemistry

Journal: Life

Article Title: Dementia Is Induced via the AGEs/Iba1/iNOS Pathway in Aged KK-Ay/Tajcl Mice

doi: 10.3390/life13071540

Figure Lengend Snippet: Effect of aging on the levels of Iba1 ( A ), CCR7 ( B ), iNOS ( C ), TNF-α ( D ), and NO ( E ) in the hippocampus of the KK-Ay/TaJcl mice. The data show one representative experiment containing five animals. The hippocampus levels of Iba1, CCR7, iNOS, and TNF-α in the mice were measured using an ELISA kit. The hippocampus level of NO was measured using the assay kit. Values are expressed as the mean ± the SD derived from five animals. * p < 0.05, ** p < 0.01. Control: C57BL/6j mice.

Article Snippet: ELISA kits were used to measure the hippocampus concentrations of AGEs, tumor necrosis factor (TNF)-α, RAGE (MyBioSource, San Diego, CA, USA), cluster of differentiation (CD) 163 (Elabscience, Houston, TX, USA), ionized calcium binding adapter molecule 1 (Iba1: South San Francisco, CA, USA), CC-chemokine receptor 7 (CCR7: abbexa, Cambridge, UK), inducible nitric oxide synthase (iNOS), and arginase-1 (CUSABIO, Houston, TX, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Biomedicines

Article Title: Skin, Liver, and Kidney Interactions Contribute to Skin Dryness in Aging KK-Ay/Tajcl Mice

doi: 10.3390/biomedicines10102648

Figure Lengend Snippet: Effect of aging on ROS levels in the skin ( A ) and liver ( B ); and the expression of Iba1( C ) and CCR7 ( D ) in KK-Ay/TaJcl mice compared with the control. Western blot diagram of Iba1, CCR7, and β-actin with molecular weight markers ( E ). We measured the levels of ROS using a commercial kit and Western blotting for the analysis of Iba1 and CCR7. Values are expressed as the mean ± SD derived from five animals. * p < 0.05. Control: C57BL/6j mice.

Article Snippet: The membranes were washed with Tris-buffered saline–0.1% Tween 20 three times and were incubated at room temperature for 1 h with primary antibodies against Iba1 (marker of macrophage; 1:1000; Wako), chemokine receptor 7 (CCR7) (marker of M1 macrophage; 1:1000; Abcam, Cambridge, MA, USA), and β-actin as a loading control (1:5000; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Expressing, Western Blot, Molecular Weight, Derivative Assay